intact cells  (Eppendorf AG)

Journal: Physiological Reports

Article Title: E‐cigarette exposure impairs skeletal muscle mitochondrial function in male mice

doi: 10.14814/phy2.70797

Figure Lengend Snippet: Tobacco smoke and E‐cig vapor condensates activate the AHR pathway and alter mitochondrial respiration in cultured muscle cells. (a) C2C12 myotubes were treated for 16 h without affecting cell viability. (b–d) mRNA expression of Ahr , Ahrr , and Cyp1a1 in treated myotubes. (e) Schematic overview of mitochondrial assay. (f, g) Mitochondrial respiration assessed using respirometry. Data analyzed using Kruskal‐Wallis test. N = 4–6 biological replicates per group. Error bars represent the standard deviation.

Article Snippet: First, intact cell respiration was measured, followed by the addition of digitonin (10 μg/mL; ThermoFisher Scientific; Cat. No. BN2006) to permeabilize the myotubes.

Techniques: Cell Culture, Expressing, Standard Deviation

Journal: Physiological Reports

Article Title: E‐cigarette exposure impairs skeletal muscle mitochondrial function in male mice

doi: 10.14814/phy2.70797

Figure Lengend Snippet: Chronic tobacco smoke or E‐cig exposure reduces skeletal muscle mitochondrial respiration but not contractile function in male mice. (a) Graphical depiction of experimental groups and exposure time. (b) Body weight. (c) Mass of hindlimb muscles normalized by body weight. (d) Schematic overview of mitochondrial assay. (e–h) Mitochondrial respiratory function. (i) Schematic overview of in situ muscle function testing. (j–k) Plantar flexor muscle contractile strength measured in situ. Data analyzed using Kruskal–Wallis test. N = 4 per group. Error bars represent the standard deviation.

Article Snippet: First, intact cell respiration was measured, followed by the addition of digitonin (10 μg/mL; ThermoFisher Scientific; Cat. No. BN2006) to permeabilize the myotubes.

Techniques: Muscles, In Situ, Standard Deviation